Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: H33_parsnp3

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	H33_parsnp3
Reference	Subramanyam S et al. (2016) Targeted Discovery of Single-Nucleotide Polymorphisms in an Unmarked Wheat Chromosomal Region Containing the Hessian Fly Resistance Gene H33 Crop Science 56:1106-1114.
General Remarks	Amplicon size of flanking sequence = 390
Type	KASP
PCR primers	SNP flanking forward GGTGGATGGACCGGACGCTC SNP Flanking reverse AACGCTCGCAAATGGCCACC
PCR primers	KASP forward resistant TTCTGAAAGTGGTCTGCAGATTGC KASP forward susceptible TTCTGAAAGTGGTCTGCAGATTGT KASP common reverse GCAGCATCGTAAGATGGGGAAGAAT
Amplification Conditions	Anneal flanking primers at 58C
Source Gene	H33 (Triticum)