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GrainGenes Probe Report: H33_parsnp2

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	H33_parsnp2
Reference	Subramanyam S et al. (2016) Targeted Discovery of Single-Nucleotide Polymorphisms in an Unmarked Wheat Chromosomal Region Containing the Hessian Fly Resistance Gene H33 Crop Science 56:1106-1114.
General Remarks	Amplicon size of flanking sequence = 329
Type	KASP
PCR primers	SNP flanking forward CCCGTGCATATCCTATTATCCTCTGGC SNP Flanking reverse ACCTGAGTCACGGCCTGCG
PCR primers	KASP forward resistant GGAATTAGGTCTGAAGGAAGCAAAGA KASP forward susceptible GAATTAGGTCTGAAGGAAGCAAAGG KASP common reverse CGGTGCTCATCTTCTGCTTTTCCTT
Amplification Conditions	Anneal flanking primers at 63C
Source Gene	H33 (Triticum)