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GrainGenes Probe Report: PLATZ-A1_4bpDel

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	PLATZ-A1_4bpDel
Reference	Zhang J et al. (2023) Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1) Proceedings of the National Academy of Sciences, USA 120. Dubcovsky J MAS Wheat. Bringing Genomics to the Wheat Fields. Plant height gene Rht25. MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks	'4-bp deletion in the third exon of PLATZ-A1 found in Chinese Spring (CS RefSeq v1.1, a CAGG deletion between 6A:145, 651, 864 and 145, 651, 865, on the - strand)' See the MASWheat webpage for amplification conditions and note that those conditions should be considered only as a starting point of the method optimization at individual laboratories.
Type	KASP
PCR primers	Plz-4bp-wt-FAM 5'- GAAGGTGACCAAGTTCATGCTACATGTGACAGGATCCTCcag -3' Plz-4bp-mut-VIC 5'- GAAGGTCGGAGTCAACGGATTACATGTGACAGGATCCTCAGC -3' Plz-del-common 5'- TCCGACGGGACAATTAGGC -3'