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GrainGenes Probe Report: PLATZ-A1_19bpDel

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
PLATZ-A1_19bpDel
Reference
ReferenceZhang J et al. (2023) Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1) Proceedings of the National Academy of Sciences, USA 120.
ReferenceDubcovsky J MAS Wheat. Bringing Genomics to the Wheat Fields. Plant height gene Rht25. MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks
'19-bp deletion and the second one is a 19-bp deletion (CS RefSeq v1.1, 6A:145, 651, 892 -145, 651, 910). It is present in McNeal'
See the MASWheat webpage for amplification conditions and note that those conditions should be considered only as a starting point of the method optimization at individual laboratories.
Type
KASP
PCR primers
Plz-19bp-wt-FAM 5'- GAAGGTGACCAAGTTCATGCTcctcagtccaggccattc -3'
Plz-19bp-mut-VIC 5'- GAAGGTCGGAGTCAACGGATTcctcagtccaggccgtct -3'
Plz-del-common 5'- TCCGACGGGACAATTAGGC -3'