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GrainGenes Probe Report: G2373A

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	G2373A
Locus	TaGW2-6A
Reference	Simmonds J et al. (2016) A splice acceptor site mutation in TaGW2-A1 increases thousand grain weight in tetraploid and hexaploid wheat through wider and longer grains. Theoretical and Applied Genetics 129:1099-112. MAS Wheat. Bringing Genomics to the Wheat Fields. Yield. Thousand Grain Weight - Grain Size. TaGW2-A1 MAS Wheat. Marker Assisted Selection in Wheat.
Type	KASP
PCR primers	F_TaGW2-6A 5'-AGA GCA ATT TGT AAG TCT TAT TCC -3' R_TaGW2-6A_Mutant 5'-GCT TCA ATG ACT TTC TGT TCT TCT-3' F_TaGW2-6A 5'-AGA GCA ATT TGT AAG TCT TAT TCC -3' R_TaGW2-6A_Wildtype 5'-GCT TCA ATG ACT TTC TGT TCT TCC-3'
Amplification Conditions	Hotstart at 95C for 15 min Touchdown (10 cycles): 95C, 20 sec; 65C, -1C per cycle, 25 sec; 72C, 45 sec. Amplification (26 cycles): 95C, 10 sec; 57C, 60 sec
Specificity	G2373A SNP [G/A] transition in splice acceptor site of exon 5 of TaGW2-6A identified in TILLING mutant lines. A-type mutation results in frame-shift generating a premature termination codon yielding a shorter protein of 134 amino acids. Lines possessing this mutation showed increased thousand grain weight (TKW) and grain width (GW). Reverse primers are for wild type and mutant type, respectively.
Sequence	KP749902
Source Gene	TaGW-A2