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GrainGenes Probe Report: FSD_RSA

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	FSD_RSA
Locus	SrCad
Reference	Laroche A et al. (2000) Development of a PCR marker for rapid identification of the Bt-10 gene for common bunt resistance in wheat Genome 43:217-223. Hiebert C and Fetch T MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stem Rust Resistance. SrCad MAS Wheat. Marker Assisted Selection in Wheat.
PCR primers	FSD 5'- GTT TTA TCT TTT TAT TTC RSA 5'- CTC CTC CCC CCA
Amplification Conditions	Denaturing step: 94C, 3 min. Amplification step (35 cycles): 94C, 30 sec; 44C, 1 min 45 sec; 72C, 2 min. Extension step: 72C, 10 min