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GrainGenes Probe Report: SUN209

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	SUN209
Locus	Sr49 Xsun209 Sr56
Reference	Bansal UK et al. (2015) Mapping of a new stem rust resistance gene Sr49 in chromosome 5B of wheat Theoretical and Applied Genetics 128:2113-2119. Bansal U et al. (2014) Molecular mapping of an adult plant stem rust resistance gene Sr56 in winter wheat cultivar Arina Theoretical and Applied Genetics 127:1441-1448. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stem Rust Resistance. Sr49 MAS Wheat. Marker Assisted Selection in Wheat. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stem Rust Resistance. Sr56 MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR SSR
PCR primers	Sr49: sun209-F 5- AGA CTA TGA GCT TCG CTA TTG sun209-R 5- GTG ATT GGT TCG GAT TAC TTA Sr56: sun209-F 5'- CTG TAA GGT TCT TTC GGA TTG G sun209-R 5'- CAT GGT CTT CGA CGA CTT AGT G
Amplification Conditions	Sr49: Denaturing step: 95C, 10 min. Amplification step (35 cycles): 92C, 30 sec; 60C, 40 sec; 72C, 50 sec. Extension step: 72C, 7 min Sr56: Denaturing step: 95 C, 10 min. Amplification step (35 cycles): 95C, 15 sec; 60C, 30 sec; 72C, 30 sec