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GrainGenes Probe Report: SUN479

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	SUN479
Locus	Sr49 Xsun479
Reference	Bansal UK et al. (2015) Mapping of a new stem rust resistance gene Sr49 in chromosome 5B of wheat Theoretical and Applied Genetics 128:2113-2119. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stem Rust Resistance. Sr49 MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	sun479-F 5- CAA ATG AAA TGT GAT CCT GTT sun479-R 5- TCA TCT AAC CAG CAA TGG TAT
Amplification Conditions	Denaturing step: 95C, 10 min. Amplification step (35 cycles): 92C, 30 sec; 60C, 40 sec; 72C, 50 sec. Extension step: 72C, 7 min