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GrainGenes Probe Report: BE404728

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	BE404728
Locus	Sr44 XBE404728
Reference	Liu W et al. (2013) Development and characterization of a compensating wheat-Thinopyrum intermedium Robertsonian translocation with Sr44 resistance to stem rust (Ug99). Theoretical and Applied Genetics 126:1167-1177. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stem Rust Resistance. Sr44 MAS Wheat. Marker Assisted Selection in Wheat.
Type	CAPS
PCR primers	be404728-F 5'- GGT GGT GCC TGT CAA GAT T be404728-R 5'- TTG ATG GAT CCT GGC TTA GG
Amplification Conditions	Denaturing step: 94C, 10 min. Touchdown step (decrease 0.5C/cycle for 10 cycles): 94C, 20 sec; 63-58C, 45 sec; 72C, 120 sec. Amplification step (35 cycles): 94C, 20 sec; 58C, 20 sec; 72C, 120 sec. Extensions step: 72C, 10 min