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GrainGenes Probe Report: RWGS30

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	RWGS30
Locus	Sr43 Xrwgs30
Reference	Niu Z et al. (2014) Development and characterization of wheat lines carrying stem rust resistance gene Sr43 derived from Thinopyrum ponticum. Theoretical and Applied Genetics 127:969-980. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stem Rust Resistance. Sr43 MAS Wheat. Marker Assisted Selection in Wheat.
Type	EST
PCR primers	rwgs30-F 5'- CTC TTG GTG CCA CAC TCT GA rwgs30-R 5'- TCA GTT CCC TCC CAT TCA TC
Amplification Conditions	Denaturing step: 95C, 5 min. Amplification step (45 cycles): 95C, 40 sec; 56C, 40 sec; 72C, 1 min. Extension step: 72C, 10 min