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GrainGenes Probe Report: Sr39-22r

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	Sr39-22r
Locus	Sr39
Reference	Mago R et al. (2009) Development of wheat lines carrying stem rust resistance gene Sr39 with reduced Aegilops speltoides chromatin and simple PCR markers for marker-assisted selection Theoretical and Applied Genetics 119:1441-1450. Mago R and Dundas I MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr39 MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	5'- AGA GAA GAT AAG CAG TAA ACA TG -3' 5'- TGC TGT CAT GAG AGG AAC TCT G -3'
Amplification Conditions	Denaturing step: 94C, 5 min. Amplification step (30 cycles): 92C, 30 sec; 58C, 30 sec; 72C, 40 sec. Extension step: 72C, 10 min