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GrainGenes Probe Report: STM773-2

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	STM773-2
Locus	Sr36
Reference	Tsilo TJ et al. (2008) Diagnostic Microsatellite Markers for the Detection of Stem Rust Resistance Gene Sr36 in Diverse Genetic Backgrounds of Wheat Crop Science 48:253-261. Tsilo TJ et al. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr36 MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
Amplification Conditions	Denaturing step: 94C, 10 min. Touchdown step 1 (7 cycles): 92C, 1 min; 64C, 1 min; 72C, 1 min. Touchdown step 2 (5 cycles): 92C, 1 min; 57C, 1 min; 72C, 1 min. Amplification step (10-25 cycles): 92C, 30 sec; 55C, 1 min; 72C, 1 min. Extension step: 72C, 10 min