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GrainGenes Probe Report: csSr32-2

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	csSr32-2
Locus	Sr32
Reference	Mago R et al. (2013) Development of wheat–Aegilops speltoides recombinants and simple PCR-based markers for Sr32 and a new stem rust resistance gene on the 2S-1 chromosome Theoretical and Applied Genetics 126:2943-2955. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Leaf Rust Resistance. Lr32 MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	5'- CAA ATG AAT AGA AAA ACC CGT GCT -3' 5'- CAC ACA CTG TTT TCC GTT GC -3'
Amplification Conditions	Denaturing step: 95C, 2 min. Amplification step (30 cycles): 95C, 30 sec; 60C, 40 sec; 72C, 50 sec. Extension step: 72C, 5 min