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GrainGenes Probe Report: Gb

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	Gb
Locus	Sr25
Reference	Yu LX et al. (2010) Haplotype diversity of stem rust resistance loci in uncharacterized wheat lines Molecular Breeding 26:667-680. Yu LX et al. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr25 MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	Gb-F 5'- CAT CCT TGG GGA CCT C -3' Gb-R 5'- CCA GCT CGC ATA CAT CCA -3'
Amplification Conditions	Denaturing step: 94C, 4 min. Amplification step (35 cycles): 94C, 45 sec; 50C, 30 sec; 72C, 45 sec. Extension step: 72C, 7 min