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GrainGenes Probe Report: Sr24-12

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	Sr24-12
Locus	Sr24
Reference	Mago R et al. (2005) Development of PCR markers for the selection of wheat stem rust resistance genes Sr24 and Sr26 in diverse wheat germplasm Theoretical and Applied Genetics 111:496-504. Lowe IZ and Soria MA MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr24 MAS Wheat. Marker Assisted Selection in Wheat.
Type	AFLP PCR
PCR primers	5'- CAC CCG TGA CAT GCT CGT A -3' 5'- AAC AGG AAA TGA GCA ACG ATG T -3'
Amplification Conditions	Denaturing step: 94C, 5 min. Touchdown step 1 (7 cycles, 1C down each cycle): 92C, 30 sec; 62C, 30 sec;72C, 30 sec. Amplification step (30 cycles): 92C, 30 sec; 59C, 30 sec; 72C, 30 sec. Extension step: 72C, 10 min