Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: EX594406

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	EX594406
Locus	Sr21
Reference	Chen S et al. (2015) Fine mapping and characterization of Sr21, a temperature-sensitive diploid wheat resistance gene effective against the Puccinia graminis f. sp. tritici Ug99 race group Theoretical and Applied Genetics 128:645-656. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr21 MAS Wheat. Marker Assisted Selection in Wheat.
Type	CAPS PCR
PCR primers	EX594406-F 5’- TCA ACA ACT TCA ACA AGG C -3´ EX594406-R 5’- AAC AAG AGA ACG AGC ATC G -3´
Amplification Conditions	Denaturing step: 94C, 5 min. Amplification step (40 cycles): 94C, 30 sec; 52C, 30 sec; 72C, 60 sec. Extension step: 72C, 7 min