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GrainGenes Probe Report: FD527726

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	FD527726
Locus	Sr21
Reference	Chen S et al. (2015) Fine mapping and characterization of Sr21, a temperature-sensitive diploid wheat resistance gene effective against the Puccinia graminis f. sp. tritici Ug99 race group Theoretical and Applied Genetics 128:645-656. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr21 MAS Wheat. Marker Assisted Selection in Wheat.
Type	CAPS PCR
PCR primers	FD527726-F 5´- CGG CAT CAA TAG GAG AAG A -3´ FD527726-R 3´- TAG GAT ACG TGA CCC AGG A -3´
Amplification Conditions	Denaturing step: 94C, 5 min. Amplification step (40 cycles): 94C, 30 sec; 53C, 30 sec; 72C, 60 sec. Extension step: 72C, 7 min