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GrainGenes Probe Report: SUN104

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	SUN104
Locus	Yr51 Xsun104
Reference	Randhawa M et al. (2014) Molecular mapping of strip rust resistance gene Yr51 in chromosome 4AL of wheat Theoretical and Applied Genetics 127:317-324. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stripe Rust Resistance. Yr51 MAS Wheat. Marker Assisted Selection in Wheat.
Type	STS
PCR primers	sun104-F 5'- TGC TAT GTG CGT GAT GAT GA sun104-R 5'- TTA CAT GCT CCA GCG ACT TG
Amplification Conditions	Denaturing step: 95C, 10 min. Touchdown step (decrease 1C/cycle for 6 cycles): 92C, 30 sec; 65-60C, 30 sec; 72C, 30 sec. Amplification step (34 cycles): 92C, 30 sec; 60C, 30 sec; 72C, 30 sec. Extensions step: 72C, 7 min
Source Gene	Yr51 (Triticum)