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GrainGenes Probe Report: csGS

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	csGS
Locus	Lr68
Reference	Herrera-Foessel SA et al. (2012) Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat Theoretical and Applied Genetics 124:1475-1486. Herrera-Foessel S et al. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Leaf Rust Resistance. Lr68 MAS Wheat. Marker Assisted Selection in Wheat.
Type	STS
PCR primers	csGS-F1 5'- AAG ATT GTT CAC AGA TCC ATG TCA csGS-R1 5'- GAG TAT TCC GGC TCA AAA AGG
Amplification Conditions	Denaturing step: 93C, 1 min. Amplification step (30 cycles): 93C, 30 sec, 60C (csGS and cs7BLNLRR), 1min, 72C, 1 min. Extensions step: 72C, 5 min