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GrainGenes Probe Report: Pinb-D1b

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	Pinb-D1b
Locus	Pin-b
Reference	Giroux MJ and Morris CF (1997) A glycine to serine change in puroindoline b is associated with wheat grain hardness and low levels of starch-surface friabilin. Theoretical and Applied Genetics 95:857-864. MAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
PCR primers	5' Pinb_F: ATGAAGACCTTATTCCTCCTA 3' 5' Pinb_R: CTCATGCTCACAGCCGCT 3'
Specificity	Specific to allele Pinb-D1b for the puroindoline Pinb-D1 gene. PCR primers produce a 250 bp fragment in the presence of the Pinb-D1b allele.