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GrainGenes Probe Report: GLU B3bef

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
GLU B3bef
Locus
Glu-B3
Reference
ReferenceWang LH et al. (2009) Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.). Theoretical and Applied Genetics 118:525-539.
ReferenceMAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Type
PCR
STS
PCR primers
5' SB10F: GCATCAACAACAAATAGTACTAGAA 3'
5' SB10R: GGCGGGTCACACATGACA 3'
Specificity
Specific to Low molecular weight (LMW) glutenin Glu-B3 alleles Glu-B3b, Glu-B3e, and Glu-B3f. Primer pair produced a 750 bp fragment in the presence of the Glu-B3a, Glu-B3e, and Glu-B3f alleles.