Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: GLU A3ac

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	GLU A3ac
Locus	Glu-A3
Reference	Wang LH et al. (2010) Development of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.) Journal of Cereal Science 51:305-312. MAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR STS
PCR primers	5' LA1F: AAACAGAATTATTAAAGCCGG 3' 5' SA3R: GTGGCTGTTGTGAAAACGA 3'
Specificity	Specific for Low molecular weight (LMW) glutenin Glu-A3 alleles Glu-A3a and Glu-A3c. Primer pair produced a 573 bp fragment in the presence of the Glu-A3a allele and Glu-A3c allele.