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GrainGenes Probe Report: GLU A3e

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	GLU A3e
Locus	Glu-A3
Reference	Wang LH et al. (2010) Development of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.) Journal of Cereal Science 51:305-312. MAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR STS
PCR primers	5' LA1F: AAACAGAATTATTAAAGCCGG 3' 5' SA5R: GGCACAGACGAGGAAGGTT 3'
Specificity	Specific for Low molecular weight (LMW) glutenin Glu-A3 allele Glu-A3e. Primer pair produced a 158 bp fragment in the presence of the Glu-A3e allele.