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GrainGenes Probe Report: GLU D1d

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	GLU D1d
Locus	Glu-D1
Reference	Ishikawa G and Nakamura T (2007) A new co-dominant PCR-based marker to identify the high-molecular-weight glutenin subunit combination (5+10) of common wheat. Wheat Information Service 103:1-3. MAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	5' Dx_F: TTTGGGGAATACCTGCACTACTAAAAAGGT 3' 5' Dx5_F: AAAAGGTATTACCCAAGTGTAACTTGTCCG 3' 5' Dx_R: AATTGTCCTGGCTGCAGCTGCGA 3'
Amplification Conditions	Denaturing step; 95C, 5 min. Amplification step (32 cycles): 95C, 30 sec; 65C, 30 sec; 72C, 2 min. Extension step: 72C, 7 min. 4C to end
Specificity	Specific for High molecular weight (HMW) glutenin Glu-D1 subunit combination Dx5 and Dy10 resulting from the Glu-D1d allele. PCR primers produced two fragments of 343 bp and 320 bp when the Glu-D1d allele was present, and a single 361 bp fragment when the allele was absent.