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GrainGenes Probe Report: GLU Dy-10/12

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	GLU Dy-10/12
Locus	Glu-D1
Reference	Smith R et al. (1994) Identification of glutenin alleles in wheat and triticale using PCR generated DNA markers Crop Science 34:1373-1378. MAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	5' Dy_F: GTTGGCCGGTCGGCTGCCATG 3' 5' R: Dy_TGGAGAAGTTGGATAGTACC 3'
Specificity	Specific for High molecular weight (HMW) glutenin Glu-D1 subunits Dy10 and Dy12. Primers targeting the Glu-D1 locus resulted in a marker for which the Dy10 genotype produced a 576 bp fragment and the Dy12 genotype produced a 612 bp fragment.