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GrainGenes Probe Report: UMN26

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	UMN26
Locus	Glu-D1 umn26
Reference	Liu S et al. (2008) New DNA markers for high molecular weight glutenin subunits in wheat. Theoretical and Applied Genetics 118:177-183. MAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	5' UMN26F: CGCAAGACAATATGAGCAAACT 3' 5' UMN26R: TTGCCTTTGTCCTGTGTGC 3'
Specificity	Specific for High molecular weight (HMW) glutenin Glu-D1 subunits Dy10 and Dy12. Primers targeting the Glu-D1 locus resulted in a marker for which the Dy10 genotype produced a 397 bp fragment and the Dy12 genotype produced a 415 bp fragment.
Source Species	Avena sativa