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GrainGenes Probe Report: UMN25

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	UMN25
Locus	Glu-D1 umn25
Reference	Liu S et al. (2008) New DNA markers for high molecular weight glutenin subunits in wheat. Theoretical and Applied Genetics 118:177-183. MAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
PCR primers	5' UMN25F: GGGACAATACGAGCAGCAAA 3' 5' UMN25R: CTTGTTCCGGTTGTTGCCA 3'
Specificity	Specific for High molecular weight (HMW) glutenin Glu-D1 subunits Dx2 and Dx5. PCR primers produced a 299 bp fragment in the presence of the Dx2 subunit and a 281 bp fragment in the presence of the Dx5 subunit
Source Species	Avena sativa