Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: ZSBy9aF1/R3

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	ZSBy9aF1/R3
Locus	Glu-B1
Reference	Lea ZS et al. (2006) Y-type gene specific markers for enhanced discrimination of high-molecular weight glutenin alleles at the Glu-B1 locus in hexaploid wheat Journal of Cereal Science 43:94-101. MAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	5' By9_F: TTCTCTGCATCAGTCAGGA 3' 5' By9_R: AGAGAAGCTGTGTAATGCC 3'
Specificity	Specific for High molecular weight (HMW) glutenin Glu-B1 subunit By9 produced by the Glu-B1-2b allele. PCR primers produced 662 bp fragment when the Glu-B1-2b allele was present, and a 707 bp fragment when the allele was absent.