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GrainGenes Probe Report: UMN19

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	UMN19
Locus	Glu-A1 umn19
Reference	Liu S et al. (2008) New DNA markers for high molecular weight glutenin subunits in wheat. Theoretical and Applied Genetics 118:177-183. MAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	5' UMN19F: CGAGACAATATGAGCAGCAAG 3' 5' UMN19R: CTGCCATGGAGAAGTTGGA 3'
Specificity	Specific for High molecular weight (HWM) glutenin Glu-A1 subunits Ax2* and Ax1. PCR primers produced a 344 bp fragment for Ax2* genotype, 362 bp fragment for Ax1 and Ax-null genotypes.
Source Species	Avena sativa