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GrainGenes Probe Report: YP7A-2

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
YP7A-2
Locus
Psy-A1
Reference
ReferenceHe XY et al. (2009) Allelic variants of phytoene synthase 1 ( Psy1 ) genes in Chinese and CIMMYT wheat cultivars and development of functional markers for flour colour Molecular Breeding 23:553-563.
ReferenceMAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Type
PCR
PCR primers
5' F: GCCAGCCCTTCAAGGACATG 3'
5' R: CAGATGTCGCCACACTGCCA 3'
Specificity
PCR primers amplified a 1686 bp fragment in the presence of the Psy1-A1a allele, a 1686 bp fragment in the presence of the Psy1-A1b allele, and a 1001 bp fragment in the presence of the PsyA1c allele.