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GrainGenes Probe Report: PPO33

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	PPO33
Locus	Ppo-A1
Reference	He XY et al. (2007) Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat. Theoretical and Applied Genetics 115:47. MAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR STS
PCR primers	5' F: CCAGATACACAACTGCTGGC 3' 5' R: TGATCTTGAGGTTCTCGTCG 3'
Specificity	Specific to polyphenol oxidase gene Ppo-A1. PCR primers amplify a 290 bp fragment in the the presense of the high PPO activity genotype, and 481 bp fragment in the presence of the low PPO activity genotype.