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GrainGenes Probe Report: cssfr6

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	cssfr6
Locus	Lr34
Reference	Lagudah ES et al. (2009) Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theoretical and Applied Genetics 119:889-898. MAS Wheat. Functional Markers for disease resistance genes in wheat MAS Wheat. Marker Assisted Selection in Wheat. Soria MA et al. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Leaf Rust Resistance. Lr34-Yr18 MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR SNP
PCR primers	5' cssfr6_f: CTGAGGCACTCTTTCCTGTACAAAG 3' 5' cssfr6_r: GCATTCAATGAGCAATGGTTATC 3'
Specificity	Specific for leaf rust resistance gene Lr34. PCR primers produced a 652 bp fragment in the presence of Lr34 and a 649 bp fragment in the absence of Lr34.