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GrainGenes Probe Report: cssfr2

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	cssfr2
Locus	Lr34
Reference	Lagudah ES et al. (2009) Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theoretical and Applied Genetics 119:889-898. MAS Wheat. Functional Markers for disease resistance genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	5' L34DINT9F: TTGATGAAACCAGTTTTTTTTCTA 3' 5' L34MINUSR: TATGCCATTTAACATAATCATGAA 3'
Specificity	Specific for the absence of the leaf rust resistance gene Lr34. PCR primers produced a 1170 bp fragment in the absence of Lr34.