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GrainGenes Probe Report: Pm3e

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	Pm3e
Locus	Pm3
Reference	Tommasini L et al. (2006) Development of functional markers specific for seven Pm3 resistance alleles and their validation in the bread wheat gene pool. Theoretical and Applied Genetics 114:165. MAS Wheat. Functional Markers for disease resistance genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	5' Pm3e/F: GGAATCCCTTTGGCTTGT 3' 5' Pm3e/R: CTAGCAGAGCAGTGCAAG 3'
Specificity	Specific for powdery mildew resistance gene Pm3 allele Pm3e. PCR primers produce a 524 bp fragment in the presence of the Pm3e allele.