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GrainGenes Probe Report: GLU Bx-7/17

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	GLU Bx-7/17
Locus	Glu-B1
Reference	MAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	5' Bx_F: CGCAACAGCCAGGACAATT 3' 5' Bx_R: AGAGTTCTATCACTGCCTGGT 3'
Specificity	Specific for High molecular weight (HMW) glutenin Glu-B1 Bx7 and Bx17 subunits. Primers targeting the Glu-B1 locus resulted in a co-dominant marker for which the Bx7 genotype produced two fragments (630 bp and 766 bp) and the Bx17 genotype a single fragment (669 bp).