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GrainGenes Probe Report: WMC581

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC581  [ Marker Report ]
Locus	Xwmc581-7B Xwmc581 wmc581 wmc581-7B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 15h3.2 Donor: Aventis Multiple inserts in this clone, this is the second insert. Trimmed to insert at both ends. Bases 104 to 161 (complement) show homology to 6g11 bases 194 to 251 (58 in longest run, maxScore = 55). (GT)23 36 to 81, (GT)8 83 to 98. Reverse primer matches 6g11 sequence. Foward primer is unique.
Type	PCR SSR
PCR primers	Forward cATgTTgccATcAAAcTcgc at 10 length 20 bases, Tm 64 Reverse gctaTTgAcATgcAAcTATggAcct at 108 length 25 bases, Tm 64
SSR size	123 bases expected, Chinese Spring
Sequence	WMC581
Source Species	Triticum aestivum