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GrainGenes Probe Report: WMC576

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC576
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 15g2 Donor: Aventis Trimmed to insert at both ends. Bases 471 to 497 (complement) show homology to 13h6 bases 10 to 36 (27 in longest run, maxScore = 113). (GA)13 169 to 194, (GA)27 266 to 319. Forward primer has 8 mismatches with 13h6, reverse primer has one mismatch.
Type	PCR SSR
PCR primers	Forward gcATAcAATgAgTgcAcccAA at 43 length 21 bases, Tm 64 Reverse tTgATgcATAAAAcgcgAccT at 341 length 21 bases, Tm 64
SSR size	319 bases expected, Chinese Spring
Sequence	WMC576
Source Species	Triticum aestivum