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GrainGenes Probe Report: WMC566

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC566
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14c9 Donor: Weining, Queensland Reverse sequence only. Trimmed to insert at 5' end, 3' end not located. Bases 48 to 79 (complement) show homology to 11d11 bases 195 to 226 (32 in longest run, maxScore = 61). (GT)8 214 to 229, (GT)5 162 to 171. Reverse primer has 2 mismatches to 11d11, forward primer has 8 mismatches.
Type	PCR SSR
PCR primers	Forward gcATTTggcAggcATcAT at 13 length 18 bases, Tm 63 Reverse ATTTccTcAAAcAccccg at 241 length 18 bases, Tm 60
SSR size	246 bases expected, Chinese Spring
Amplification Conditions	Moomorphic at 61C anneal.
Sequence	WMC566
Source Species	Triticum aestivum