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GrainGenes Probe Report: WMC565

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC565
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 14a9
Donor: Weining, Queensland
Reverse sequence only. Trimmed to insert at 5' end, 3' end not located. Bases 99 to 115 (complement) show homology to 19g4 bases 56 to 72 (17 in longest run, maxScore = 47). (CA)9 331 to 348, (CT)9 168 to 185, (CA)7 1 to 14, (CA)7 281 to 294.
Type
PCR
SSR
PCR primers
Forward gAAcAcccgcTTgTTTATcTcc at 50 length 22 bases, Tm 64
Reverse TcAAgAccAAAgTgTcAcccTg at 228 length 22 bases, Tm 64
SSR size
200 bases expected, Chinese Spring
Amplification Conditions
Monomorphic at 61C anneal.
Sequence
WMC565
Source Species
Triticum aestivum