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GrainGenes Probe Report: WMC564

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC564
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14g8 Donor: Weining, Queensland Reverse sequence only. Trimmed to insert at 5' end, 3' end not located. (GT)64 60 to 187.
Type	PCR SSR
PCR primers	Forward ATTcATTAcgggAAAcAccTgg at 18 length 22 bases, Tm 64 Reverse AgggTTATTAcTgccgTggAcT at 211 length 22 bases, Tm 63
SSR size	215 bases expected, Chinese Spring
Amplification Conditions	No amplification at 61C anneal. Monomorphic at 51C anneal.
Sequence	WMC564
Source Species	Triticum aestivum