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GrainGenes Probe Report: WMC563

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC563
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14d8 Donor: Weining, Queensland Consensus sequence shown. Trimmed to Rsa1 at 3' end, 5' end not located. (CA)8 27 to 42, (CA)18 45 to 80, (CA)8 83 to 98, (CA)5 4 to 13.
Type	PCR SSR
PCR primers	Forward cAcATgggTggggAAcg at 10 length 17 bases, Tm 64 Reverse ccAggggAgAcAAggcA at 123 length 17 bases, Tm 63
SSR size	130 bases expected, Chinese Spring
Amplification Conditions	Weak smear at 61C anneal. Polymorphic at 51C anneal, though bands smaller than expected.
Sequence	WMC563
Source Species	Triticum aestivum