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GrainGenes Probe Report: WMC562

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC562
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14d7 Donor: Weining, Queensland Trimmed reverse sequence only. Bases 133 to 149 show homology to 3g6 bases 217 to 233 (17 in longest run, maxScore = 21). (CA)11 95 to 116, (CA)5 29 TO 38, (CA)5 120 to 129
Type	PCR SSR
PCR primers	Forward AcggTgggAATcccTgTTT at 2 length 19 bases, Tm 63 Reverse cccAgcATgTTTgTgTgAg at 142 length 19 bases, Tm 61
SSR size	159 bases expected, Chinese Spring
Amplification Conditions	No amplification 61C anneal. Monomorphic at 51C anneal.
Sequence	WMC562
Source Species	Triticum aestivum