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GrainGenes Probe Report: WMC560

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC560
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10e4 Donor: Gale, John Innes Sequence received 14.9.99. Forward sequence only. Trimmed to Rsa1 at 5' end, 3' end not located. (GT)28 39 to 94.
Type	PCR SSR
PCR primers	Forward gccgTTggATcTTggTTAcATT at 12 length 22 bases, Tm 64 Reverse cAcAgTAAccAAAggAAAgccc at 163 length 22 bases, Tm 64
SSR size	173 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC560
Source Species	Triticum aestivum