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GrainGenes Probe Report: WMC557

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC557  [ Marker Report ]
Locus	Xwmc557-7B Yr59 wmc557
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stripe Rust Resistance. Yr59 MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26b12 Donor: Momont Trimmed to insert at both ends. (GCC)6 218 to 235.
Type	PCR SSR
PCR primers	Forward ggTgcTTgTTcATAcgggcT at 44 length 20 bases, Tm 64 Reverse AggTccTcgATccgcTcAT at 323 length 19 bases, Tm 64
SSR size	298 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (140 bases, 280-320). Gives nulls for 140.
Sequence	WMC557
Source Gene	Yr59 (Triticum)
Source Species	Triticum aestivum