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GrainGenes Probe Report: WMC552

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC552  [ Marker Report ]
Locus	Xwmc552-3D [ Show all 7 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26e10 Donor: Momont Trimmed to insert at both ends. (GT)25 91 to 140.
Type	PCR SSR
PCR primers	Forward AcTAAggAgTgTgAgggcTgTg at 33 length 22 bases, Tm 62 Reverse cTcTcgcgcTATAAAAgAAggA at 160 length 22 bases, Tm 62
SSR size	149 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (140-160 bases).
Sequence	WMC552
Source Species	Triticum aestivum