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GrainGenes Probe Report: WMC550

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC550
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26g9 Donor: Momont Trimmed to Ssp1 at 5' end and to insert at 3' end. (CA)15 174 to 203.
Type	PCR SSR
PCR primers	Forward gAcccTgTgcTgcTATggAT at 81 length 20 bases, Tm 62 Reverse gccAccccTggTgAATTTAc at 241 length 20 bases, Tm 63
SSR size	180 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (166-174 bases).
Sequence	WMC550
Source Species	Triticum aestivum