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GrainGenes Probe Report: WMC549

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC549  [ Marker Report ]
Locus	Xwmc549-3D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26e9 Donor: Momont Trimmed to insert at both ends. Bases 77 to 98 show homology to 6b12 bases 205 to 226 (22 in longest run, maxScore = 73), forward primer no obvious match to 6b12, reverse primer has 3 mismatches.. (CA)4 52 to 59, (CA)5 61 to 70, (CA)7 73 to 86.
Type	PCR SSR
PCR primers	Forward TTgTcAcAcAcgcAcTccc at 11 length 19 bases, Tm 64 Reverse gTccTTcccTcgTTcATccT at 176 length 20 bases, Tm 63
SSR size	185 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (144-202 bases), complex .
Sequence	WMC549
Source Species	Triticum aestivum