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GrainGenes Probe Report: WMC543

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC543
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10d2.2 Donor: Gale, John Innes Multiple inserts in this clone, this is the second insert. Trimmed to Rsa1 at 5' end 3' end not located. (GA)9 91 to 108.
Type	PCR SSR
PCR primers	Forward AccAcAAATcATATAATcgcAc at 32 length 22 bases, Tm 58 Reverse TAccTTTcccTcTTATgTccTT at 223 length 22 bases, Tm 58
SSR size	213 bases expected, Chinese Spring
Amplification Conditions	51C anneal, complex product.
Sequence	WMC543
Source Species	Triticum aestivum