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GrainGenes Probe Report: WMC542

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC542
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10c5 Donor: Gale, John Innes Trimmed to insert at both ends. Bases 157 to 179 show homology to 6b1 bases 191 to 213 (23 in longest run, maxScore = 98). Forward primer has 3 mismatches to 6b1, no obvious match for reverse primer. (CA)8 197 to 212, (CA)10 264 to 283, (CT)9 246 to 263.
Type	PCR SSR
PCR primers	Forward cAATAAAATTAcTcTcTccc at 77 length 20 bases, Tm 50 Reverse TgAAAgcTAgAgTTTggT at 283 length 18 bases, Tm 50
SSR size	224 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (very complex) .
Sequence	WMC542
Source Species	Triticum aestivum