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GrainGenes Probe Report: WMC541

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC541
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10c4 Donor: Gale, John Innes Trimmed to Rsa1 at 3' end, 5' end not located. Bases 100 to 140 (complement) show homology to 22c5 bases 356 to 396 (41 in longest run, maxScore = 87), classified as duplicate of 22c5, 2c5 has priority . (AC)7 264 to 277.
Type	PCR SSR
PCR primers	Forward TTccTcgTTcATccTTTTcTcT at 37 length 22 bases, Tm 61 Reverse gTgTTTATgTgAgTgTTgcgTg at 298 length 22 bases, Tm 61
SSR size	283 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC541
Source Species	Triticum aestivum